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| Title: | | CRYSTAL STRUCTURE OF AN ADENINE BULGE IN THE RNA CHAIN OF A DNA/RNA HYBRID, D(CTCCTCTTC)/R(GAAGAGAGAG) | |
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| Molecular Description: | 5'-D(CpTpCpCpTpCpTpTpC)-3'
5'-R(GpApApGpApGpApGpApG)-3' |
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| Structural Features: | A DOUBLE HELIX, FLIPPED-OUT BASES |
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| Nucleic Acid Sequence: |
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| Chain | A: | |
(DC) (DT) (DC) (DC) (DT) (DC) (DT) (DT) (DC)
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| Chain | B: | |
G A A G A G A G A G
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| Primary Citation: | Sudarsanakumar, C., Xiong, Y., Sundaralingam, M.
Crystal structure of an adenine bulge in the RNA chain of a DNA.RNA hybrid, d(CTCCTCTTC).r(gaagagagag).
J.Mol.Biol.
, 299,
pp. 103 - 112, 2000.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
I
2
2
2
|
|
| Cell Constants: |
| a = 46.66 | b = 47.61 | c = 54.04 | (Ångstroms) |
 = 90.0 |  = 90.0 |  = 90.0 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | SPERMINE TETRACHLORIDE, CACODYLATE, LiCl, MPD |
| Reservoir: | | LICL, MPD |
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| Refinement: | The structure was refined using the CNS program.
The R value is
19.9
for 2486 reflections
in the resolution range 10.0 to
2.30 Ångstroms
with I > 1.0 sigma(I).
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