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| Title: | | THE CRYSTAL STRUCTURE OF AN RNA/DNA HYBRID REVEALS NOVEL INTERMOLECULAR INTERCALATION | |
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| Molecular Description: | 5'-R(CpApApApGpApApApApG)-3'
5'-D(CpTpTpTpTpCpTpTpTpG)-3' |
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| Structural Features: | A DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Chain | A: | |
C A A A G A A A A G
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| Chain | B: | |
(DC) (DT) (DT) (DT) (DT) (DC) (DT) (DT) (DT) (DG)
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| Primary Citation: | Han, G.W., Kopka, M.L., Langs, D., Sawaya, M.R., Dickerson, R.E.
Crystal structure of an RNADNA hybrid reveals intermolecular intercalation: Dimer formation by base-pair swapping
Proc.Natl.Acad.Sci.USA
, 100,
pp. 9214 - 9219, 2003.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
I
2
2
2
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|
| Cell Constants: |
| a = 45.234 | b = 46.780 | c = 56.560 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | MPD, magnesium acetate, spermidine, sodium cacodylate |
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| Refinement: | The structure was refined using the CNS program.
The R value is
22.3
for 2171 reflections
in the resolution range 10 to
2.38 Ångstroms
with Fobs > 2.0 sigma(Fobs).
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