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| Title: | | MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGMO6AATTCGCG): N6-METHOXYADENOSINE/ THYMIDINE BASE-PAIRS IN B-DNA | |
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| Molecular Description: | 5'-D(CpGpCpGp(A47)pApTpTpCpGpCpG)-3' |
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| Structural Features: | B DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Chains | A, B: | |
(DC) (DG) (DC) (DG) (A47) (DA) (DT) (DT) (DC) (DG) (DC) (DG)
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| Primary Citation: | Chatake, T., Hikima, T., Ono, A., Ueno, Y., Matsuda, A., Takenaka, A.
Crystallographic studies on damaged DNAs. II. N(6)-methoxyadenine can present two alternate faces for Watson-Crick base-pairing, leading to pyrimidine transition mutagenesis.
J.Mol.Biol.
, 294,
pp. 1223 - 1230, 1999.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
21
21
21
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| Cell Constants: |
| a = 24.9 | b = 39.5 | c = 66.2 | (Ångstroms) |
 = 90.0 |  = 90.0 |  = 90.0 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | MPD, SODIUM CACODYLATE, MAGNESIUM ACETATE, NaCl, SPERMINE |
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| Refinement: | The structure was refined using the X-PLOR 3.843 program.
The R value is
23.2
for 3303 reflections
in the resolution range 5.0 to
2.0 Ångstroms
with Fobs > 3.0 sigma(Fobs).
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