 | | | |
| Title: | | DNA DECAMER WITH AN ENGINEERED CROSSLINK IN THE MINOR GROOVE | |
|
| Molecular Description: | 5'-D(CpCpApGp(G47)pCpCpTpGpG)-3' |
 |
| Structural Features: | B DOUBLE HELIX |
|
| Nucleic Acid Sequence: |
| | | |
| Chains | A, B, C, D: | |
(DC) (DC) (DA) (DG) (G47) (DC) (DC) (DT) (DG) (DG)
|
|
|
| Primary Citation: | van Aalten, D.M., Erlanson, D.A., Verdine, G.L., Joshua-Tor, L.
A structural snapshot of base-pair opening in DNA.
Proc.Natl.Acad.Sci.USA
, 96,
pp. 11809 - 11814, 1999.
|
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
21
21
21
|
|
| Cell Constants: |
| a = 34.435 | b = 43.180 | c = 72.412 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
|
|
| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | CaCl2, NaCl, SPERMINE, SODIUM CACODYLATE, MPD |
| Reservoir: | | MPD |
|
|
| Refinement: | The structure was refined using the CNS program.
The R value is
20.3
for 15525 reflections
in the resolution range 13.0 to
1.55 Ångstroms
with I > 0.0 sigma(I).
|
|
|
