NDB ID: BDF062
Title: BASE-PAIR OPENING AND SPERMINE BINDING-B-DNA FEATURES DISPLAYED IN THE CRYSTAL STRUCTURE OF A GAL OPERON FRAGMENT: IMPLICATIONS FOR PROTEIN-DNA RECOGNITION Molecular Description: 5'-D(CpGpGpTpGpG)-3' SPERMINE Structural Features: B DOUBLE HELIX, OPEN BASE PAIR Nucleic Acid Sequence: Chain A: (DC) (DG) (DG) (DT) (DG) (DG) Chain B: (DC) (DC) (DA) (DC) (DC) (DG) Primary Citation: Tari, L.W., Secco, A.S. Base-pair opening and spermine binding--B-DNA features displayed in the crystal structure of a gal operon fragment: implications for protein-DNA recognition. Nucleic Acids Res. , 23, pp. 2065 - 2073, 1995. Experimental Information: X-RAY DIFFRACTION Space Group: P  61   2  2 Cell Constants: a = 54.450 b = 54.450 c = 42.100 (Ångstroms) = 90.00 = 90.00 = 120.00 (degrees) Crystallization Conditions: Method: VAPOR DIFFUSION, HANGING DROP Drop: WATER, MPD, MgCl2, SPERMINE, Na Cacodylate Reservoir: WATER, MPD Refinement: The structure was refined using the X-PLOR program. The R value is 22.1 for 392 reflections in the resolution range 20.000 to 2.500 Ångstroms with Fobs > 1.000 sigma(Fobs).	Biological Unit 1 Other Views Asymmetric Unit Crystal Packing Enlarge Biological Unit 1
Coordinate + Structure Data Asymmetric Unit coordinates (pdb format, Unix compressed(.gz)) Asymmetric Unit coordinates (cif format, Unix compressed(.gz)) Biological Unit coordinates (pdb format) XML | Complete with coordinates (xml format, GNU compressed(.gz)) XML | Coordinates only (xml format, GNU compressed(.gz)) XML | Header only (xml format, GNU compressed(.gz))	Derivative Data Nucleic Acid Backbone Torsions Base Pair Parameters Base Pair Step Parameters Hydrogen Bonding Classification
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The Protein Data Bank: 206D
PubMed: 7596838
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