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| Title: | | MOLECULAR STRUCTURE OF NICKED DNA. MODEL A4 | |
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| Molecular Description: | 5'-D(CpGpCpGpApApApApCpGpCpG)-3'/ 5'-D(
CpGpCpGpTpT)-3'+ 5'-D(TpTpCpGpCpG)-3'
MODEL A4 |
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| Structural Features: | B DOUBLE HELIX, NICKED |
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| Nucleic Acid Sequence: |
| | | |
| Chain | A: | |
C G C G A A A A C G C G
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| Chain | B: | |
C G C G T T
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| Chain | C: | |
T T C G C G
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| Primary Citation: | Aymani, J., Coll, M., Van Der Marel, G.A., Van Boom, J.H., Wang, A.H.-J., Rich, A.
Molecular Structure of Nicked DNA: A Substrate for DNA Repair Enzymes
Proc.Natl.Acad.Sci.USA
, 87,
pp. 2526 - 2530, 1990.
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| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
21
21
21
|
|
| Cell Constants: |
| a = 25.990 | b = 44.030 | c = 66.620 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION |
| Drop: | | WATER, MPD, MgCl2, Na Cacodylate, Spermine HCl |
| Reservoir: | | WATER, MPD |
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| Refinement: | The structure was refined using the NUCLSQ program.
The R value is
18.2
for 553 reflections
in the resolution of 3.000
with Fobs > 2.000 sigma(Fobs).
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