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| Title: | | STRUCTURE OF THE TOPOISOMERASE II POISON BOUND TO DNA | |
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| Molecular Description: | 5'-D(CpGpTpApCpG)-3'
9-AMINO-[N-(2-DIMETHYLAMINO)ETHYL]ACRIDINE-4- CARBOXAMIDE |
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| Structural Features: | RH DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Chain | A: | |
(DC) (DG) (DT) (DA) (DC) (DG)
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| Primary Citation: | Adams, A., Guss, J.M., Collyer, C.A., Denny, W.A., Wakelin, L.P.
Crystal structure of the topoisomerase II poison 9-amino-[N-(2-dimethylamino)ethyl]acridine-4-carboxamide bound to the DNA hexanucleotide d(CGTACG)2.
Biochemistry
, 38,
pp. 9221 - 9233, 1999.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
64
|
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| Cell Constants: |
| a = 30.161 | b = 30.161 | c = 39.692 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | SODIUM CACODYLATE, MAGNESIUM ACETATE, COCL2, SPERMINE, MPD |
| Reservoir: | | MPD |
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| Refinement: | The structure was refined using the SHELXL-97 program.
The R value is
19.34
for 2705 reflections
in the resolution range 16.0 to
1.6 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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