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| Title: | | CRYSTAL STRUCTURE OF A BIOTIN-BINDING RNA PSEUDOKNOT | |
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| Molecular Description: | BIOTIN-BINDING RNA PSEUDOKNOT
BIOTIN |
 |
| Structural Features: | LOOP, INTERNAL LOOP, HAIRPIN LOOP, BASE TRIPLET |
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| Nucleic Acid Sequence: |
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| Chain | A: | |
A C C G U C A G A G G A C A C G G U U
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| Chain | B: | |
A A A A A G U C C U C
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| Primary Citation: | Nix, J., Sussman, D., Wilson, C.
The 1.3 A crystal structure of a biotin-binding pseudoknot and the basis for RNA molecular recognition.
J.Mol.Biol.
, 296,
pp. 1235 - 1244, 2000.
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| | | |
| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
|
|
| Cell Constants: |
| a = 47.59 | b = 30.96 | c = 62.11 | (Ångstroms) |
 = 90 |  = 111.88 |  = 90 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | POTASIUM HEPES, MgCl2, KCl, BIOTIN, SPERMINE, MPD |
| Reservoir: | | MPD |
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| Refinement: | The structure was refined using the CNS program.
The R value is
19.9
for 20852 reflections
in the resolution range 17.18 to
1.30 Ångstroms
with I > 1.0 sigma(I).
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