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| Title: | | D34G MUTANT OF PVUII ENDONUCLEASE COMPLEXED WITH COGNATE DNA SHOWS THAT ASP34 IS DIRECTLY INVOLVED IN DNA RECOGNITION AND INDIRECTLY INVOLVED IN CATALYSIS | |
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| Molecular Description: |
PROTEIN (PVUII ENDONUCLEASE) MUTANT (D34G)/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chains | C, D: | |
(DT) (DG) (DA) (DC) (DC) (DA) (DG) (DC) (DT) (DG) (DG) (DT) (DC)
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| Protein Sequence: |
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| Primary Citation: | Horton, J.R., Nastri, H.G., Riggs, P.D., Cheng, X.
Asp34 of PvuII endonuclease is directly involved in DNA minor groove recognition and indirectly involved in catalysis.
J.Mol.Biol.
, 284,
pp. 1491 - 1504, 1998.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
21
21
21
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| Cell Constants: |
| a = 95.800 | b = 86.300 | c = 48.500 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Refinement: | The structure was refined using the X-PLOR program.
The R value is
20.5
for 50527 reflections
in the resolution range 5.0 to
1.59 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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