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| Title: | | STRUCTURES OF SAP-1 BOUND TO DNA SEQUENCES FROM THE E74 AND C-FOS PROMOTERS PROVIDE INSIGHTS INTO HOW ETS PROTEINS DISCRIMINATE BETWEEN RELATED DNA TARGETS | |
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| Molecular Description: | SAP-1 ETS DOMAIN/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chain | A: | |
(DT) (DA) (DC) (DC) (DG) (DG) (DA) (DA) (DG) (DT)
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| Chain | B: | |
(DA) (DA) (DC) (DT) (DT) (DC) (DC) (DG) (DG) (DT)
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| Protein Sequence: |
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| Primary Citation: | Mo, Y., Vaessen, B., Johnston, K., Marmorstein, R.
Structures of SAP-1 bound to DNA targets from the E74 and c-fos promoters: insights into DNA sequence discrimination by Ets proteins.
Mol.Cell
, 2,
pp. 201 - 212, 1998.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
21
1
|
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| Cell Constants: |
| a = 31.590 | b = 55.670 | c = 44.230 | (Ångstroms) |
 = 90.00 |  = 107.16 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Drop: | | SODIUM CACODYLATE, PEG
8000 ZINC ACETATE |
| Reservoir: | | PEG
8000 |
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| Refinement: | The structure was refined using the X-PLOR 3.851 program.
The R value is
22
for 10700 reflections
in the resolution range 28.9 to
1.93 Ångstroms
with Fobs > 2.0 sigma(Fobs).
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