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| Title: | | ANALYSIS OF A MUTATIONAL HOT-SPOT IN THE ECORV RESTRICTION ENDONUCLEASE: A CATALYTIC ROLE FOR A MAIN CHAIN CARBONYL GROUP | |
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| Molecular Description: |
RESTRICTION ENDONUCLEASE ECORV (E.C.3.1.21.4)/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX, FLIPPED-OUT BASES |
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| Nucleic Acid Sequence: |
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| Chains | C,D: | | (DA) (DA) (DA) (DG) (DA) (DT) (DA) (DT) (DC) (DT) (DT) |
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| Protein Sequence: |
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| Primary Citation: | Thomas, M.P., Brady, R.L., Halford, S.E., Sessions, R.B., Baldwin, G.S.
Structural analysis of a mutational hot-spot in the EcoRV restriction endonuclease: a catalytic role for a main chain carbonyl group.
Nucleic Acids Res.
, 27,
pp. 3438 - 3445, 1999.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
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| Cell Constants: |
| a = 48.350 | b = 48.780 | c = 63.640 | (Ångstroms) |
 = 96.61 |  = 108.94 |  = 107.44 | (degrees) |
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| Refinement: | The structure was refined using the REFMAC program.
The R value is
18.9
for 21703 reflections
in the resolution range 12.50 to
2.30 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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