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| Title: |  | THE CRYSTAL STRUCTURE OF A POST-REACTIVE COGNATE DNA-ECO RI COMPLEX AT 2.50 A IN THE PRESENCE OF MN2+ ION |  |
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| Molecular Description: | ENDONUCLEASE ECORI/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASE |
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| Nucleic Acid Sequence: |
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| Chain | M: | | (DT) (DC) (DG) (DC) (DG) |
| Chain | N: | | (DA) (DA) (DT) (DT) (DC) (DG) (DC) (DG) |
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| Protein Sequence: |
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| Primary Citation: | Horvath, M.M., Choi, J., Kim, Y., Wilkosz, P., Rosenberg, J.M. The Integration of Recognition and Cleavage: X-Ray Structures of Pre- Transition State and Post-Reactive DNA-Eco RI Endonuclease Complexes
To be Published |
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
3
2
1
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| Cell Constants: |
| a = 118.4 | b = 118.4 | c = 49.7 | (Ångstroms) |
= 90. | = 90. | = 120. | (degrees) |
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| Refinement: | The structure was refined using the X-PLOR 3.1 program.
The R value is
17.7
for 7596 reflections
in the resolution range 8.0 to
2.5 Ångstroms
with Fobs > 3. sigma(Fobs).
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