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| Title: | | ECORV BOUND TO MN2+ AND COGNATE DNA CONTAINING A 3'S SUBSTITION AT THE CLEAVAGE SITE | |
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| Molecular Description: |
TYPE II RESTRICTION ENZYME ECORV (E.C.3.1.21.4)/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chains | C,D: | | (DC) (DA) (DA) (DG) (DA) (TSP) (DA) (DT) (DC) (DT) (DT) |
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| Protein Sequence: |
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| Primary Citation: | Horton, N.C., Connolly, B.A., Perona, J.J.
Inhibition of EcoRV Endonuclease by Deoxyribo-3'-S-phosphorothiolates: A High-Resolution X-ray Crystallographic Study
J.Am.Chem.Soc.
, 122,
pp. 3314 - 3324, 2000.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
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| Cell Constants: |
| a = 47.7 | b = 48.5 | c = 63.7 | (Ångstroms) |
 = 96.7 |  = 109.6 |  = 106.3 | (degrees) |
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| Refinement: | The structure was refined using the X-PLOR program.
The R value is
21.3
for 35951 reflections
in the resolution range 4.90 to
1.90 Ångstroms
with I > 1. sigma(I).
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