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| Title: | | ECORV BOUND TO 3'-S-PHOSPHOROTHIOLATE DNA AND CA2+ | |
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| Molecular Description: |
TYPE II RESTRICTION ENZYME ECORV (E.C.3.1.21.4)/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chain | C: | | (DA) (DA) (DA) (DG) (DA) (TSP) (DA) (DT) (DC) (DT) (DT) |
| Chain | D: | | (DC) (DA) (DA) (DG) (DA) (TSP) (DA) (DT) (DC) (DT) (DT) |
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| Protein Sequence: |
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| Primary Citation: | Horton, N.C., Connolly, B.A., Perona, J.J.
Inhibition of EcoRV Endonuclease by Deoxyribo-3'-S-phosphorothiolates: A High-Resolution X-ray Crystallographic Study
J.Am.Chem.Soc.
, 122,
pp. 3314 - 3324, 2000.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
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| Cell Constants: |
| a = 47.9 | b = 48.6 | c = 63.9 | (Ångstroms) |
 = 96.9 |  = 108.9 |  = 106.8 | (degrees) |
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| Refinement: | The structure was refined using the X-PLOR program.
The R value is
22.9
for 61223 reflections
in the resolution range 4.80 to
1.60 Ångstroms
with I > 1. sigma(I).
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