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| Title: |  | L116A MUTANT OF THE HOMING ENDONUCLEASE I-PPOI COMPLEXED TO HOMING SITE DNA. |  |
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| Molecular Description: | I-PPOI HOMING ENDONUCLEASE/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Protein Sequence: |
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| Primary Citation: | Galburt, E.A., Chadsey, M.S., Jurica, M.S., Chevalier, B.S., Erho, D., Tang, W., Monnat Jr., R.J., Stoddard, B.L.
Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site.
J.Mol.Biol.
, 300,
pp. 877 - 887, 2000.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 182.000 | b = 73.100 | c = 92.700 | (Ångstroms) |
= 90.00 | = 95.40 | = 90.00 | (degrees) |
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| Refinement: | The structure was refined using the X-PLOR 3.851 program.
The R value is
27.3
for 19707 reflections
in the resolution range 50.0 to
3.1 Ångstroms
with I > 0. sigma(I).
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