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| Title: | | PVUII ENDONUCLEASE/COGNATE DNA COMPLEX (GLUTARALDEHYDE-CROSSLINKED CRYSTAL) AT PH 7.5 WITH TWO CALCIUM IONS AT EACH ACTIVE SITE | |
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| Molecular Description: |
TYPE II RESTRICTION ENZYME PVUII (E.C.3.1.21.4)/DNA COMPLEX |
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| Structural Features: | B double helix |
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| Nucleic Acid Sequence: |
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| Chains | C,D: | | (DT) (DG) (DA) (DC) (DC) (DA) (DG) (DC) (DT) (DG) (DG) (DT) (DC) |
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| Protein Sequence: |
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| Primary Citation: | Horton, J.R., Cheng, X.
PvuII endonuclease contains two calcium ions in active sites.
J.Mol.Biol.
, 300,
pp. 1049 - 1056, 2000.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
21
21
21
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| Cell Constants: |
| a = 94.043 | b = 84.702 | c = 45.993 | (Ångstroms) |
 = 90. |  = 90. |  = 90. | (degrees) |
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| Refinement: | The structure was refined using the X-PLOR 3.851 program.
The R value is
20.5
for 12739 reflections
in the resolution range 30.0 to
2.5 Ångstroms
with I > 0. sigma(I).
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