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| Title: |  | CRYSTAL STRUCTURE OF CENP-B(1-129) COMPLEXED WITH THE CENP-B BOX DNA |  |
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| Molecular Description: |
CENP-B DNA BINDING DOMAIN/CENP-B BOX DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Protein Sequence: |
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| Primary Citation: | Tanaka, Y., Nureki, O., Kurumizaka, H., Fukai, S., Kawaguchi, S., Ikuta, M., Iwahara, J., Okazaki, T., Yokoyama, S.
Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA.
EMBO J.
, 20,
pp. 6612 - 6618, 2001.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
31
1
2
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| Cell Constants: |
| a = 83.374 | b = 83.374 | c = 139.021 | (Ångstroms) |
= 90 | = 90 | = 120 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | MPD, potassium cacodylate, MgCl2 |
| Reservoir: | | MPD |
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| Refinement: | The structure was refined using the CNS program.
The R value is
22.19
for 19313 reflections
in the resolution range 30 to
2.5 Ångstroms
with I > 0.0 sigma(I).
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