 | | | |
| Title: | | ANTIBODY 64M-2 FAB COMPLEXED WITH DTT(6-4)TT | |
|
| Molecular Description: |
ANTI-(6-4) PHOTOPRODUCT ANTIBODY 64M-2 FAB (LIGHT CHAIN)/ANTI-(6-4) PHOTOPRODUCT ANTIBODY 64M-2 FAB (HEAVY CHAIN)/DNA Complex |
 |
| Structural Features: | SINGLE STRAND |
|
| Nucleic Acid Sequence: |
| | | |
| Chain | A: | |
(DT) (64T) (DT) (DT)
|
|
|
| Protein Sequence: |
|
|
| Primary Citation: | Yokoyama, H., Mizutani, R., Satow, Y., Sato, K., Komatsu, Y., Ohtsuka, E., Nikaido, O.
Crystal structure of DNA (6-4) photoproduct dTT(6-4)TT in complex with the 64M-2 antibody Fab fragment implies increased antibody-binding affinity by the flanking nucleotides
To be Published |
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
21
21
21
|
|
| Cell Constants: |
| a = 73.567 | b = 137.274 | c = 49.615 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
|
|
| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
8000 NiCl2, Tris-HCl |
|
|
| Refinement: | The structure was refined using the X-PLOR 3.843 program.
The R value is
20.1
for 17221 reflections
in the resolution range 10.0 to
2.40 Ångstroms
with Fobs > 0 sigma(Fobs).
|
|
|
