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| Title: |  | ANTIBODY 64M-2 FAB COMPLEXED WITH DTT(6-4)TT |  |
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| Molecular Description: |
ANTI-(6-4) PHOTOPRODUCT ANTIBODY 64M-2 FAB (LIGHT CHAIN)/ANTI-(6-4) PHOTOPRODUCT ANTIBODY 64M-2 FAB (HEAVY CHAIN)/DNA Complex |
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| Structural Features: | SINGLE STRAND |
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| Nucleic Acid Sequence: |
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| Chain | A: | |
(DT) (64T) (DT) (DT)
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| Protein Sequence: |
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| Primary Citation: | Yokoyama, H., Mizutani, R., Satow, Y., Sato, K., Komatsu, Y., Ohtsuka, E., Nikaido, O. Crystal structure of DNA (6-4) photoproduct dTT(6-4)TT in complex with the 64M-2 antibody Fab fragment implies increased antibody-binding affinity by the flanking nucleotides
To be Published |
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
21
21
21
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| Cell Constants: |
| a = 73.567 | b = 137.274 | c = 49.615 | (Ångstroms) |
= 90.00 | = 90.00 | = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
8000 NiCl2, Tris-HCl |
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| Refinement: | The structure was refined using the X-PLOR 3.843 program.
The R value is
20.1
for 17221 reflections
in the resolution range 10.0 to
2.40 Ångstroms
with Fobs > 0 sigma(Fobs).
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