 | | | |
| Title: | | BASIS FOR A SWITCH IN SUBSTRATE SPECIFICITY: CRYSTAL STRUCTURE OF SELECTED VARIANT OF CRE SITE-SPECIFIC RECOMBINASE, LNSGG BOUND TO THE ENGINEERED RECOGNITION SITE LOXM7 | |
|
| Molecular Description: | Recombinase CRE/DNA Complex |
|
| Structural Features: | B DOUBLE HELIX, OPEN |
|
| Nucleic Acid Sequence: |
|
|
| Protein Sequence: |
|
|
| Primary Citation: | Baldwin, E.P., Martin, S.S., Abel, J., Gelato, K.A., Kim, H., Schultz, P.G., Santoro, S.W.
A specificity switch in selected cre recombinase variants is mediated by macromolecular plasticity and water.
Chem.Biol.
, 10,
pp. 1085 - 1094, 2003.
|
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
C
2
2
21
|
|
| Cell Constants: |
| a = 107.410 | b = 121.350 | c = 180.610 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
|
|
| Refinement: | The structure was refined using the TNT program.
The R value is
22.4
for 24813 reflections
in the resolution range 5.0 to
2.75 Ångstroms
with Fobs > 0. sigma(Fobs).
|
|
|
