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| Title: |  | THE PHIX174 DNA BINDING PROTEIN J IN TWO DIFFERENT CAPSID ENVIRONMENTS. |  |
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| Molecular Description: |
Capsid protein/Major spike protein/Small core protein/DNA Complex |
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| Structural Features: | SINGLE STRAND |
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| Nucleic Acid Sequence: |
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| Chain | X: | |
(DC) (DA) (DA) (DA)
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| Protein Sequence: |
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| Primary Citation: | Bernal, R.A., Hafenstein, S., Esmeralda, R., Fane, B.A., Rossmann, M.G.
The phiX174 Protein J Mediates DNA Packaging and Viral Attachment to Host Cells.
J.Mol.Biol.
, 337,
pp. 1109 - 1122, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
H
3
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| Cell Constants: |
| a = 295.143 | b = 295.143 | c = 678.235 | (Ångstroms) |
= 90.00 | = 90.00 | = 120.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | PEG
8000 sodium citrate, glycerol, sodium azide, beta-mercapto-ethanol, NaCl, water |
| Reservoir: | | PEG
8000 sodium citrate, glycerol, NaCl |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
23.4
for 269234 reflections
in the resolution range 92.91 to
3.50 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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