NDB ID: PD0525


Title:TERNARY 3' COMPLEX OF T7 DNA POLYMERASE WITH A DNA PRIMER/TEMPLATE CONTAINING A DISORDERED CIS-SYN THYMINE DIMER ON THE TEMPLATE AND AN INCOMING NUCLEOTIDE
Molecular Description:
DNA POLYMERASE(E.C.2.7.7.7)/THIOREDOXIN 1/DNA Complex
Structural Features:A DOUBLE HELIX, overhanging base
Nucleic Acid Sequence:
ChainsP,Q:Click here for sequence.
ChainsT,U:Click here for sequence.
Protein Sequence:
ChainsA,C: Click here for sequence.
ChainsB,D: Click here for sequence.
Primary Citation:Li, Y., Dutta, S., Doublie, S., Bdour, H.M., Taylor, J.S., Ellenberger, T.
Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7.
Nat.Struct.Mol.Biol. , 11, pp. 784 - 790, 2004.
Experimental Information:X-RAY DIFFRACTION
Space Group: 21   1
Cell Constants:
a = 54.266b = 105.466c = 213.931(Ångstroms)
= 90.00 = 91.57 = 90.00(degrees)
Refinement:The structure was refined using the CNS 1.1 program. The R value is 28.2 for 39656 reflections in the resolution range 50.0 to 3.20 Ångstroms with Fobs > 0.0 sigma(Fobs).

Biological Assembly 1
Other Views
Asymmetric Unit

Biological Assemblies
Enlarge Biological Assembly 1
Enlarge Biological Assembly 2



Coordinate + Structure Data

Asymmetric Unit coordinates (pdb format, Unix compressed(.gz))

Asymmetric Unit coordinates (cif format, Unix compressed(.gz))

Biological Assembly coordinates (pdb format)12

Structure Factors (cif format)

XML | Complete with coordinates (xml format, GNU compressed(.gz))
XML | Coordinates only (xml format, GNU compressed(.gz))
XML | Header only (xml format, GNU compressed(.gz))

Derivative Data

Nucleic Acid Backbone Torsions

Base Pair Parameters

Base Pair Step Parameters

Hydrogen Bonding Classification

Links

The RCSB Protein Data Bank: 1SL0

PubMed: 15235589




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