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| Title: | | STRUCTURE OF THE K38A MUTANT OF ECORV BOUND TO COGNATE DNA AND MN2+ | |
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| Molecular Description: |
Type II restriction enzyme EcoRV (E.C.3.1.21.4)/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chains | C,E: | | (DA) (DA) (DA) (DG) (DA) (DT) |
| Chains | D,F: | | (DA) (DT) (DC) (DT) (DT) |
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| Protein Sequence: |
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| Primary Citation: | Horton, N.C., Perona, J.J.
DNA cleavage by EcoRV endonuclease: two metal ions in three metal ion binding sites
Biochemistry
, 43,
pp. 6841 - 6857, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
21
1
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| Cell Constants: |
| a = 63.710 | b = 58.690 | c = 82.100 | (Ångstroms) |
 = 90.00 |  = 107.70 |  = 90.00 | (degrees) |
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| Refinement: | The structure was refined using the CNS program.
The R value is
20.1
for 32988 reflections
in the resolution range 20.0 to
2.10 Ångstroms
with Fobs > 2. sigma(Fobs).
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