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| Title: | | THE STRUCTURE OF K92A ECORV BOUND TO COGNATE DNA AND MG2+ | |
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| Molecular Description: |
Type II restriction enzyme EcoRV (E.C.3.1.21.4)/DNA Complex |
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| Structural Features: | RH DOUBLE HELIX, overhanging base |
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| Nucleic Acid Sequence: |
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| Chains | C,D: | | (DC) (DA) (DA) (DG) (DA) (DT) (DA) (DT) (DC) (DT) (DT) |
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| Protein Sequence: |
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| Primary Citation: | Horton, N.C., Perona, J.J.
DNA Cleavage by EcoRV Endonuclease: Two Metal Ions in Three Metal Ion Binding Sites.
Biochemistry
, 43,
pp. 6841 - 6857, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
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| Cell Constants: |
| a = 47.410 | b = 48.790 | c = 63.400 | (Ångstroms) |
 = 97.10 |  = 108.80 |  = 107.30 | (degrees) |
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| Refinement: | The structure was refined using the CNS program.
The R value is
19.7
for 43796 reflections
in the resolution range 20. to
1.8 Ångstroms
with I > 2. sigma(I).
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