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| Title: | | K38A ECORV BOUND TO CLEAVED DNA AND MN2+: P1 CRYSTAL FORM | |
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| Molecular Description: | Type II restriction enzyme EcoRV/5'-D(Ap
ApApGpApT)-3'/5'-D(pApTpCpTpT)-3' |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chains | C,E: | | (DA) (DA) (DA) (DG) (DA) (DT) |
| Chains | D,F: | | (DA) (DT) (DC) (DT) (DT) |
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| Protein Sequence: |
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| Primary Citation: | Horton, N.C., Perona, J.J.
DNA cleavage by EcoRV endonuclease: Two metal ions in three metal ion binding sites
Biochemistry
, 43,
pp. 6841 - 6857, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
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| Cell Constants: |
| a = 47.708 | b = 48.627 | c = 63.544 | (Ångstroms) |
 = 96.68 |  = 108.94 |  = 107.33 | (degrees) |
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| Refinement: | The structure was refined using the CNS program.
The R value is
21.4
for 74930 reflections
in the resolution range 20. to
1.5 Ångstroms
with I > 0. sigma(I).
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