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| Title: |  | ECORV BOUND TO COGNATE DNA AND MN2+ |  |
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| Molecular Description: | Type II restriction enzyme EcoRV/5'-D(C ApApGpApTpApTpCpTpT)-3' |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chains | C,D: | | (DC) (DA) (DA) (DG) (DA) (DT) (DA) (DT) (DC) (DT) (DT) |
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| Protein Sequence: |
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| Primary Citation: | Horton, N.C., Perona, J.J.
DNA Cleavage by EcoRV Endonuclease: Two Metal Ions in Three Metal Ion Binding Sites
Biochemistry
, 43,
pp. 6841 - 6857, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
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| Cell Constants: |
| a = 47.800 | b = 49.100 | c = 63.700 | (Ångstroms) |
= 96.90 | = 108.90 | = 107.10 | (degrees) |
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| Refinement: | The structure was refined using the CNS program.
The R value is
21.3
for 23851 reflections
in the resolution range 20. to
2.15 Ångstroms
with I > 0. sigma(I).
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