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| Title: | | HINCII BOUND TO CA2+ AND COGNATE DNA GTCGAC | |
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| Molecular Description: | 5'-D(GpCpCpGpGpTpCpGpApCpCpGp
G)-3'/Type II restriction enzyme HindII |
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| Structural Features: | B DOUBLE HELIX, fipped-out bases, overhanging bases |
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| Nucleic Acid Sequence: |
| | | |
| Chains | E,F,G,H: | | (DG) (DC) (DC) (DG) (DG) (DT) (DC) (DG) (DA) (DC) (DC) (DG) (DG) |
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| Protein Sequence: |
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| Primary Citation: | Etzkorn, C., Horton, N.C.
Ca2+ binding in the active site of HincII: implications for the catalytic mechanism
Biochemistry
, 43,
pp. 13256 - 13270, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
I
21
21
21
|
|
| Cell Constants: |
| a = 66.945 | b = 177.18 | c = 254.89 | (Ångstroms) |
 = 90.0 |  = 90.0 |  = 90.0 | (degrees) |
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| Refinement: | The structure was refined using the CNS program.
The R value is
18.0
for 36795 reflections
in the resolution range 20. to
2.8 Ångstroms
with I > 2. sigma(I).
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