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| Title: | | T7 DNA POLYMERASE IN COMPLEX WITH A PRIMER/TEMPLATE DNA CONTAINING A DISORDERED N-2 AMINOFLUORENE ON THE TEMPLATE, CRYSTALLIZED WITH DIDEOXY-ATP AS THE INCOMING NUCLEOTIDE. | |
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| Molecular Description: |
DNA polymerase (E.C.2.7.7.7)/Thioredoxin 1/DNA Complex |
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| Structural Features: | RH DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Protein Sequence: |
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| Primary Citation: | Dutta, S., Li, Y., Johnson, D., Dzantiev, L., Richardson, C.C., Romano, L.J., Ellenberger, T.
Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis.
Proc.Natl.Acad.Sci.Usa
, 101,
pp. 16186 - 16191, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
21
21
2
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| Cell Constants: |
| a = 105.944 | b = 213.559 | c = 52.168 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
23.0
for 50543 reflections
in the resolution range 47.68 to
2.30 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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