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| Title: | | HINCII BOUND TO CLEAVED COGNATE DNA GTCGAC AND MN2+ | |
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| Molecular Description: | 5'-D(GpCpCpGpGpTpC)-3'/ 5'-D(pGpApCpCpGp
G)-3'/ Type II restriction enzyme HincII |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES, FLIPPED-OUT BASES |
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| Nucleic Acid Sequence: |
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| Chains | E,G,I,K: | | (DG) (DC) (DC) (DG) (DG) (DT) (DC) |
| Chains | F,H,J,L: | | (DG) (DA) (DC) (DC) (DG) (DG) |
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| Protein Sequence: |
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| Primary Citation: | Etzkorn, C., Horton, N.C.
Mechanistic Insights from the Structures of HincII Bound to Cognate DNA Cleaved from Addition of Mg(2+) and Mn(2+)
J.Mol.Biol.
, 343,
pp. 833 - 849, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
I
21
21
21
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| Cell Constants: |
| a = 66.8 | b = 178.2 | c = 257.3 | (Ångstroms) |
 = 90.0 |  = 90.0 |  = 90.0 | (degrees) |
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| Refinement: | The structure was refined using the CNS program.
The R value is
19.9
for 53281 reflections
in the resolution range 50. to
2.5 Ångstroms
with Fobs > 2. sigma(Fobs).
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