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| Title: |  | E. COLI REP HELICASE/DNA COMPLEX |  |
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| Molecular Description: | REP HELICASE/DNA COMPLEX (E.C.3.6.1.-) |
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| Structural Features: | SINGLE STRAND |
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| Nucleic Acid Sequence: |
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| Chain | C: | |
(DT) (DT) (DT) (DT) (DT) (DT) (DT) (DT) (DT) (DT) (DT) (DT) (DT) (DT) (DT) (DT)
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| Protein Sequence: |
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| Primary Citation: | Korolev, S., Hsieh, J., Gauss, G.H., Lohman, T.M., Waksman, G.
Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP.
Cell
, 90,
pp. 635 - 647, 1997.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
43
21
2
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| Cell Constants: |
| a = 141.800 | b = 141.800 | c = 284.800 | (Ångstroms) |
= 90.00 | = 90.00 | = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION |
| Drop: | | WATER, NaCl, TRIS-HCL, EDTA, GLYCEROL |
| Reservoir: | | WATER, GLYCEROL |
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| Refinement: | The structure was refined using the X-PLOR 3.1 program.
The R value is
22.8
for 51675 reflections
in the resolution range 15.000 to
3.000 Ångstroms
with Fobs > 2.000 sigma(Fobs).
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