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| Title: | | HUMAN RECONSTITUTED DNA TOPOISOMERASE I IN COVALENT COMPLEX WITH A 22 BASE PAIR DNA DUPLEX | |
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| Molecular Description: |
TOPOISOMERASE I (E.C.5.99.1.2)/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Protein Sequence: |
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| Primary Citation: | Redinbo, M.R., Stewart, L., Kuhn, P., Champoux, J.J., Hol, W.G.
Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA.
Science
, 279,
pp. 1504 - 1513, 1998.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
21
1
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| Cell Constants: |
| a = 72.000 | b = 66.600 | c = 71.800 | (Ångstroms) |
 = 90.00 |  = 98.30 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | TRIS-HCL, EDTA, DTT, MgCl2, PEG
400 |
| Reservoir: | | TRIS-HCL, MgCl2, DTT, PEG
400 |
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| Refinement: | The structure was refined using the X-PLOR 3.1 program.
The R value is
24.7
for 42786 reflections
in the resolution range 20.0 to
2.1 Ångstroms
with Fobs > 2.0 sigma(Fobs).
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