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| Title: | | HOMING ENDONUCLEASE/DNA COMPLEX | |
|
| Molecular Description: |
INTRON-ENCODED ENDONUCLEASE I-PPOI/DNA COMPLEX |
 |
| Structural Features: | B DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
| | | |
| Chains | C, D: | |
(DT) (DT) (DG) (DA) (DC) (DT) (DC) (DT) (DC) (DT) (DT) (DA) (DA) (DG) (DA) (DG) (DA) (DG) (DT) (DC)
(DA)
|
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|
| Protein Sequence: |
|
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| Primary Citation: | Flick, K.E., Jurica, M.S., Monnat Jr., R.J., Stoddard, B.L.
DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI.
Nature
, 394,
pp. 96 - 101, 1998.
|
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
31
2
1
|
|
| Cell Constants: |
| a = 113.900 | b = 113.900 | c = 88.500 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
|
|
| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION |
| Drop: | | EDTA, SPERMINE, NaCl, SODIUM CITRATE, PEG
4000 |
| Reservoir: | | PEG
4000 SODIUM CITRATE, NaCl |
|
|
| Refinement: | The structure was refined using the X-PLOR 3.8 program.
The R value is
21.2
for 30671 reflections
in the resolution range 50.000 to
2.200 Ångstroms
with Fobs > 2.000 sigma(Fobs).
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