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| Title: | | STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE | |
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| Molecular Description: |
ENDONUCLEASE ECORV (E.C.3.1.21.4)/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Chains | C, D: | |
(DA) (DA) (DA) (DG) (DA) (DC) (DI) (DT) (DC) (DT) (DT)
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| Protein Sequence: |
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| Primary Citation: | Martin, A.M., Sam, M.D., Reich, N.O., Perona, J.J.
Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease.
Nat.Struct.Biol.
, 6,
pp. 269 - 276, 1999.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
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| Cell Constants: |
| a = 50.300 | b = 64.200 | c = 49.400 | (Ångstroms) |
 = 109.30 |  = 108.30 |  = 96.30 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
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| Refinement: | The structure was refined using the X-PLOR 3.1 program.
The R value is
20
for 22040 reflections
in the resolution range 6.0 to
2.15 Ångstroms
with Fobs > 1.0 sigma(Fobs).
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