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| Title: | | MG2+ BINDING TO THE ACTIVE SITE OF ECO RV ENDONUCLEASE: A CRYSTALLOGRAPHIC STUDY OF COMPLEXES WITH SUBSTRATE AND PRODUCT DNA AT 2 ANGSTROMS RESOLUTION | |
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| Molecular Description: | ECO RV (E.C.3.1.21.4)/DNA COMPLEX |
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| Structural Features: | B DOUBLE HELIX, OVERHANGING BASE, KINKED |
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| Nucleic Acid Sequence: |
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| Chains | C,E: | | (DA) (DA) (DA) (DG) (DA) (DT) |
| Chains | D,F: | | (DA) (DT) (DC) (DT) (DT) |
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| Protein Sequence: |
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| Primary Citation: | Kostrewa, D., Winkler, F.K.
Mg2+ binding to the active site of EcoRV endonuclease: a crystallographic study of complexes with substrate and product DNA at 2 A resolution.
Biochemistry
, 34,
pp. 683 - 696, 1995.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
1
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| Cell Constants: |
| a = 49.290 | b = 50.340 | c = 63.880 | (Ångstroms) |
 = 96.66 |  = 108.76 |  = 108.41 | (degrees) |
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| Refinement: | The structure was refined using the X-PLOR program.
The R value is
15.6
for 26645 reflections
in the resolution range 20.000 to
2.100 Ångstroms
with Fobs > 0.000 sigma(Fobs).
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