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| Title: | | CRYSTAL STRUCTURE OF THE LACI FAMILY MEMBER, PURR, BOUND TO DNA: MINOR GROOVE BINDING BY ALPHA HELICES | |
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| Molecular Description: | PURINE REPRESSOR/DNA COMPLEX
GUANINE |
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| Structural Features: | B DOUBLE HELIX, THE DNA IS KINKED 45 DEGREES AT THE CENTER |
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| Nucleic Acid Sequence: |
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| Chain | B: | | (DT) (DA) (DC) (DG) (DC) (DA) (DA) (DA) (DC) (DG) (DT) (DT) (DT) (DG) (DC) (DG) (DT) |
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| Protein Sequence: |
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| Primary Citation: | Lu, F., Schumacher, M.A., Arvidson, D.N., Haldimann, A., Wanner, B.L., Zalkin, H., Brennan, R.G.
Structure-based redesign of corepressor specificity of the Escherichia coli purine repressor by substitution of residue 190.
Biochemistry
, 37,
pp. 971 - 982, 1998.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
2
2
21
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| Cell Constants: |
| a = 175.920 | b = 95.050 | c = 81.590 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Refinement: | The structure was refined using the TNT program.
The R value is
22.2
for 13317 reflections
in the resolution range 10.000 to
3.000 Ångstroms
with Fobs > 1.000 sigma(Fobs).
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