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| Title: | | RNA POLYMERASE II STRAND SEPARATED ELONGATION COMPLEX, MATCHED NUCLEOTIDE | |
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| Molecular Description: |
DNA-directed RNA polymerase II largest subunit (E.C.2.7.7.6)/DNA-directed RNA polymerase II 140 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 45 kDa polypeptide (E.C.2.7.7.6)/DNA-direct |
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| Structural Features: | A DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chain | R: | |
A U C G A G A G G A
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| Chain | T: | |
(DA) (DC) (DG) (DA) (DT) (DC) (DC) (DT) (DC) (DT) (DC) (DG) (DA) (DT)
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| Protein Sequence: |
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| Primary Citation: | Westover, K.D., Bushnell, D.A., Kornberg, R.D.
Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center.
Cell (Cambridge,Mass.)
, 119,
pp. 481 - 489, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 169.647 | b = 222.338 | c = 194.316 | (Ångstroms) |
 = 90.00 |  = 101.67 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
6000 Ammonium hydrogen phosphate, sodium dihydrogen phosphate, dioxane, DTT, H2O |
| Reservoir: | | PEG
6000 Ammonium hydrogen phosphate, sodium dihydrogen phosphate, H2O |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
34.9
for 50043 reflections
in the resolution range 40 to
4.25 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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