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| Title: | | RNA POLYMERASE II STRAND SEPARATED ELONGATION COMPLEX, MISMATCHED NUCLEOTIDE | |
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| Molecular Description: |
DNA-directed RNA polymerase II largest subunit (E.C.2.7.7.6)/DNA-directed RNA polymerase II 140 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 45 kDa polypeptide (E.C.2.7.7.6)/DNA-direct |
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| Structural Features: | DOUBLE HELIX |
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| Nucleic Acid Sequence: |
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| Protein Sequence: |
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| Primary Citation: | Westover, K.D., Bushnell, D.A., Kornberg, R.D.
Structural basis of transcription: nucleotide selection by rotation in the RNA polymerase II active center.
Cell (Cambridge,Mass.)
, 119,
pp. 481 - 489, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
|
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| Cell Constants: |
| a = 168.846 | b = 222.958 | c = 193.602 | (Ångstroms) |
 = 90.00 |  = 101.17 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
6000 Ammonium hydrogen phosphate, sodium dihydrogen phosphate, dioxane, DTT, H2O |
| Reservoir: | | PEG
6000 Ammonium hydrogen phosphate, sodium dihydrogen phosphate, H2O |
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| Refinement: | The structure was refined using the CNS program.
The R value is
23
for reflections
in the resolution range 40 to
3.5 Ångstroms
. |
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