NDB ID: PH0008


Title:STRUCTURAL BASIS FOR SUBSTRATE SELECTION BY T7 RNA POLYMERASE
Molecular Description:
T7 RNA polymerase(E.C.2.7.7.6)/DNA/RNA Complex
Structural Features:B DOUBLE HELIX
Nucleic Acid Sequence:
ChainsE, H, K, N:Click here for sequence.
ChainsF, I, L, O:Click here for sequence.
ChainsG, J, M, P:Click here for sequence.
Protein Sequence:
ChainsA, B, C, D: Click here for sequence.
Primary Citation:Temiakov, D., Patlan, V., Anikin, M., McAllister, W.T., Yokoyama, S., Vassylyev, D.G.
Structural basis for substrate selection by t7 RNA polymerase.
Cell , 116, pp. 381 - 391, 2004.
Experimental Information:X-RAY DIFFRACTION
Space Group: 1
Cell Constants:
a = 81.133b = 87.703c = 206.531(Ångstroms)
= 91.93 = 91.02 = 110.66(degrees)
Refinement:The structure was refined using the CNS program. The R value is 25.5 for 73174 reflections in the resolution range 40 to 3.2 Ångstroms with Fobs > 0 sigma(Fobs).

Biological Unit 1
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Coordinate + Structure Data

Asymmetric Unit coordinates (pdb format, Unix compressed(.gz))

Asymmetric Unit coordinates (cif format, Unix compressed(.gz))

Biological Unit coordinates (pdb format)1234

Structure Factors (cif format)

XML | Complete with coordinates (xml format, GNU compressed(.gz))
XML | Coordinates only (xml format, GNU compressed(.gz))
XML | Header only (xml format, GNU compressed(.gz))

Derivative Data

Nucleic Acid Backbone Torsions

Base Pair Parameters

Base Pair Step Parameters

Hydrogen Bonding Classification

Links

The Protein Data Bank: 1S0V

PubMed: 15016373




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