NDB ID: PH0011


Title:RNA POLYMERASE II STRAND SEPARATED ELONGATION COMPLEX
Molecular Description:
DNA-directed RNA polymerase II largest subunit (E.C.2.7.7.6)/DNA-directed RNA polymerase II 140 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 45 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II

and III 27 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II

and III 23 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II

and III 14.5 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 14.2 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II

and III 8.3 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 13.6 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerases I
II

and III 7.7 kDa polypeptide (E.C.2.7.7.6)/DNA/RNA Complex
Structural Features:A DOUBLE HELIX, OVERHANGING BASES
Nucleic Acid Sequence:
ChainR:A U C G A G A G G A
ChainT:(DA) (DC) (DG) (DA) (DT) (DC) (DC) (DT) (DC) (DT) (DC) (DG) (DA) (DT)
Protein Sequence:
ChainsA, B, C, E, F, H, I, J, K, LClick here for sequences.
Primary Citation:Westover, K.D., Bushnell, D.A., Kornberg, R.D.
Structural Basis of Transcription: Separation of RNA from DNA by RNA Polymerase II
Science , 303, pp. 1014 - 1016, 2004.
Experimental Information:X-RAY DIFFRACTION
Space Group: 1
Cell Constants:
a = 167.076b = 221.256c = 193.685(Ångstroms)
= 90.00 = 100.10 = 90.00(degrees)
Refinement:The structure was refined using the CNS 1.1 program. The R value is 31.5 for 73591 reflections in the resolution range 39.86 to 3.61 Ångstroms with Fobs > 0.0 sigma(Fobs).

Biological Assembly 1
Other Views
Asymmetric Unit
RNA View
RNAML File
Enlarge Biological Assembly 1



Coordinate + Structure Data

Asymmetric Unit coordinates (pdb format, Unix compressed(.gz))

Asymmetric Unit coordinates (cif format, Unix compressed(.gz))

Biological Assembly coordinates (pdb format)

Structure Factors (cif format)

XML | Complete with coordinates (xml format, GNU compressed(.gz))
XML | Coordinates only (xml format, GNU compressed(.gz))
XML | Header only (xml format, GNU compressed(.gz))

Derivative Data

Nucleic Acid Backbone Torsions

Base Pair Parameters

Base Pair Step Parameters

Hydrogen Bonding Classification

Links

The RCSB Protein Data Bank: 1SFO

PubMed: 14963331




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