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| Title: | | RNA POLYMERASE II STRAND SEPARATED ELONGATION COMPLEX | |
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| Molecular Description: |
DNA-directed RNA polymerase II largest subunit (E.C.2.7.7.6)/DNA-directed RNA polymerase II 140 kDa polypeptide (E.C.2.7.7.6)/DNA-directed RNA polymerase II 45 kDa polypeptide (E.C.2.7.7.6)/DNA-direct |
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| Structural Features: | A DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chain | R: | |
A U C G A G A G G A
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| Chain | T: | |
(DA) (DC) (DG) (DA) (DT) (DC) (DC) (DT) (DC) (DT) (DC) (DG) (DA) (DT)
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| Protein Sequence: |
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| Primary Citation: | Westover, K.D., Bushnell, D.A., Kornberg, R.D.
Structural Basis of Transcription: Separation of RNA from DNA by RNA Polymerase II
Science
, 303,
pp. 1014 - 1016, 2004.
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| | | |
| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 167.076 | b = 221.256 | c = 193.685 | (Ångstroms) |
 = 90.00 |  = 100.10 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
6000 ammonium sodium phosphate, DTT, H2O |
| Reservoir: | | PEG
6000 ammonium sodium phosphate, DTT, H2O |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
31.5
for 73591 reflections
in the resolution range 39.86 to
3.61 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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