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| Title: | | CRYSTAL STRUCTURE OF THE PAZ DOMAIN OF HUMAN EIF2C1 IN COMPLEX WITH A 9-MER SIRNA-LIKE DUPLEX OF DEOXYNUCLEOTIDE OVERHANG | |
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| Molecular Description: |
Eukaryotic translation initiation factor 2C 1/5'-R(
CpGpUpGpApCpU)-D(pCpT)-3' |
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| Structural Features: | DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Protein Sequence: |
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| Primary Citation: | Ma, J.B., Ye, K., Patel, D.J.
Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain.
Nature
, 429,
pp. 318 - 322, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
64
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| Cell Constants: |
| a = 100.051 | b = 100.051 | c = 34.438 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | PEG
1000 potassium chloride, HEPES-NaOH, Dithiothreitol |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
22.1
for 5873 reflections
in the resolution range 19.71 to
2.60 Ångstroms
with Fobs > 0.0 sigma(Fobs).
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