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| Title: | | BACILLUS HALODURANS RNASE H CATALYTIC DOMAIN MUTANT D132N IN COMPLEX WITH 12-MER RNA/DNA HYBRID | |
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| Molecular Description: |
ribonuclease H-related protein/RNA/DNA Complex |
 |
| Structural Features: | RH DOUBLE HELIX |
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| Nucleic Acid Sequence: |
| | | |
| Chain | C: | |
G A C A C C U G A U U C
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| Chain | D: | |
(DG) (DA) (DA) (DT) (DC) (DA) (DG) (DG) (DT) (DG) (DT) (DC)
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| Protein Sequence: |
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| Primary Citation: | Nowotny, M., Gaidamakov, S.A., Crouch, R.J., Yang, W.
Crystal Structures of RNase H Bound to an RNA/DNA Hybrid: Substrate Specificity and Metal-Dependent Catalysis.
Cell (CAMBRIDGE,MASS.)
, 121,
pp. 1005 - 1016, 2005.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
|
|
| Cell Constants: |
| a = 136.393 | b = 36.964 | c = 93.202 | (Ångstroms) |
 = 90.00 |  = 121.51 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | MPD, NaCl, HEPES, H2O |
| Reservoir: | | MPD, NaCl, H2O |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
20.6
for 29836 reflections
in the resolution range 30 to
1.85 Ångstroms
with Fobs > 1 sigma(Fobs).
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