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| Title: | | B. HALODURANS RNASE H CATALYTIC DOMAIN D132N MUTANT IN COMPLEX WITH MG2+ AND RNA/DNA HYBRID (NON-P NICK AT THE ACTIVE SITE) | |
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| Molecular Description: |
RIBONUCLEASE H (E.C.3.1.26.4)/RNA/DNA complex |
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| Structural Features: | RH DOUBLE HELIX, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chain | B: | |
U C G A C A
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| Chain | C: | |
(DA) (DT) (DG) (DT) (DC) (DG)
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| Protein Sequence: |
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| Primary Citation: | Nowotny, M., Yang, W.
Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release.
Embo J.
, 25,
pp. 1924 - 1933, 2006.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 83.878 | b = 35.412 | c = 63.839 | (Ångstroms) |
 = 90.00 |  = 98.66 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | MPD, NaCl, HEPES, H2O |
| Reservoir: | | MPD, NaCl, HEPES |
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| Refinement: | The structure was refined using the CNS 1.1 program.
The R value is
22.3
for 4718 reflections
in the resolution range 30 to
2.7 Ångstroms
with I > 2.4 sigma(I).
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