NDB ID: PH0025
Title: B. HALODURANS RNASE H CATALYTIC DOMAIN E188A MUTANT IN COMPLEX WITH CA2+ AND RNA/DNA HYBRID Molecular Description: RIBONUCLEASE H (E.C.3.1.26.4)/RNA/DNA complex Structural Features: RH DOUBLE HELIX, OVERHANGING BASES Nucleic Acid Sequence: Chain B: U C G A C A Chain C: (DA) (DT) (DG) (DT) (DC) (DG) Protein Sequence: Chain A: Click here for sequence. Primary Citation: Nowotny, M., Yang, W. Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release. Embo J. , 25, pp. 1924 - 1933, 2006. Experimental Information: X-RAY DIFFRACTION Space Group: C  1  2  1 Cell Constants: a = 81.671 b = 37.091 c = 61.980 (Ångstroms) = 90.00 = 96.74 = 90.00 (degrees) Refinement: The structure was refined using the CNS 1.1 program. The R value is 21.5 for 10677 reflections in the resolution range 30. to 2.05 Ångstroms with I > 2.3 sigma(I).	Biological Assembly 1 Other Views Asymmetric Unit RNA View RNAML File Enlarge Biological Assembly 1
Coordinate + Structure Data Asymmetric Unit coordinates (pdb format, Unix compressed(.gz)) Asymmetric Unit coordinates (cif format, Unix compressed(.gz)) Biological Assembly coordinates (pdb format) Structure Factors (cif format) XML | Complete with coordinates (xml format, GNU compressed(.gz)) XML | Coordinates only (xml format, GNU compressed(.gz)) XML | Header only (xml format, GNU compressed(.gz))	Derivative Data Nucleic Acid Backbone Torsions Base Pair Parameters Base Pair Step Parameters Hydrogen Bonding Classification
Links
The RCSB Protein Data Bank: 2G8W
PubMed: 16601679
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