 | | | |
| Title: | | THREE-DIMENSIONAL STRUCTURE OF RIBONULCEASE T1 COMPLEXED WITH AN ISOSTERIC PHOSPHONATE ANALOGUE OF GPU: ALTERNATE SUBSTRATE BINDING MODES AND CATALYSIS. | |
|
| Molecular Description: | ENDORIBONUCLEASE T1/DINUCLEOTIDE COMPLEX |
 |
| Nucleic Acid Sequence: |
|
|
| Protein Sequence: |
|
|
| Primary Citation: | Arni, R.K., Watanabe, L., Ward, R.J., Kreitman, R.J., Kumar, K., Walz Jr., F.G.
Three-dimensional structure of ribonuclease T1 complexed with an isosteric phosphonate substrate analogue of GpU: alternate substrate binding modes and catalysis.
Biochemistry
, 38,
pp. 2452 - 2461, 1999.
|
|
| | | |
| Experimental Information: | X-RAY DIFFRACTION |
|
| Space Group: |
P
21
21
21
|
|
| Cell Constants: |
| a = 69.910 | b = 90.290 | c = 33.980 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
|
|
| Crystallization Conditions: |
| Drop: | | SODIUM ACETATE, TRIS, MgCl2, PEG
4000 |
| Reservoir: | | PEG
4000 |
|
|
| Refinement: | The structure was refined using the X-PLOR 3.1 program.
The R value is
18.7
for 9879 reflections
in the resolution range 8.000 to
2.000 Ångstroms
with Fobs > 2.000 sigma(Fobs).
|
|
|
