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| Title: | | CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION | |
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| Molecular Description: | RIBOSOMAL PROTEIN L25/5S RRNA COMPLEX |
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| Structural Features: | RH DOUBLE HELIX, FLIPPED-OUT BASES, OVERHANGING BASES |
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| Nucleic Acid Sequence: |
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| Chain | M: | |
C C C A U G C G A G A G U A G G G A C
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| Chain | N: | |
U G C C G A U G G U A G U G U G G G G
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| Protein Sequence: |
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| Primary Citation: | Lu, M., Steitz, T.A.
Structure of Escherichia coli ribosomal protein L25 complexed with a 5S rRNA fragment at 1.8-A resolution.
Proc.Natl.Acad.Sci.USA
, 97,
pp. 2023 - 2028, 2000.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
2
2
21
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| Cell Constants: |
| a = 75.6 | b = 76.6 | c = 95.1 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | KCl, MgCl2, CACODYLATE, MPD |
| Reservoir: | | MPD |
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| Refinement: | The structure was refined using the CNS program.
The R value is
20.7
for 21705 reflections
in the resolution range 20 to
1.8 Ångstroms
with I > 2 sigma(I).
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