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| Title: | | CRYSTAL STRUCTURE OF NOVA-2 KH3 K-HOMOLOGY RNA-BINDING DOMAIN BOUND TO 20-MER RNA HAIRPIN | |
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| Molecular Description: | RNA-BINDING PROTEIN NOVA-2/RNA |
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| Structural Features: | SINGLE STRAND, LOOP, FLIPPED-OUT BASES |
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| Nucleic Acid Sequence: |
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| Chains | C, D: | |
G A G G A C C U A G A U C A C C C C U C
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| Protein Sequence: |
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| Primary Citation: | Lewis, H.A., Musunuru, K., Jensen, K.B., Edo, C., Chen, H., Darnell, R.B., Burley, S.K.
Sequence-specific RNA binding by a Nova KH domain: implications for paraneoplastic disease and the fragile X syndrome.
Cell
, 100,
pp. 323 - 332, 2000.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
21
21
21
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| Cell Constants: |
| a = 47.850 | b = 51.210 | c = 128.600 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION |
| Drop: | | MES, (NH4)2SO4, PEG
5000 |
| Reservoir: | | (NH4)2SO4, PEG
5000 |
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| Refinement: | The structure was refined using the CNS program.
The R value is
20.8
for 12459 reflections
in the resolution range 20.0 to
2.40 Ångstroms
with I > 3.0 sigma(I).
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