1 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 GGCCACCUGA CaGUCCuCUC C 12 A2M 17 IU Crystallography ? ? Angstroms 1 G O3' -43.740 -81.620 78.607 2 G P -42.291 -80.942 78.752 O3' -40.473 -75.469 78.502 3 C P -38.916 -75.594 78.861 O3' -36.377 -71.483 80.758 4 C P -35.023 -72.143 81.316 O3' -32.320 -70.488 85.302 5 A P -31.227 -71.644 85.534 O3' -29.175 -72.423 90.288 6 C P -28.178 -73.651 89.995 O3' -27.786 -76.755 94.575 7 C P -26.902 -78.079 94.315 O3' -27.912 -82.447 95.465 8 U P -26.667 -83.347 94.985 O3' -26.985 -88.361 93.735 9 G P -25.663 -88.857 92.962 O3' -25.059 -92.320 88.327 10 A P -23.611 -92.450 87.650 O3' -22.928 -93.745 82.802 11 C P -21.334 -93.771 82.983 O3' -18.716 -91.631 78.862 12 a P -17.311 -92.104 79.486 O3' -13.017 -88.638 79.674 13 G P -11.927 -87.904 80.611 O3' -8.174 -85.798 77.358 14 U P -7.249 -85.877 78.658 O3' -4.964 -81.965 80.766 15 C P -4.073 -82.907 81.716 O3' -3.493 -80.882 86.363 16 C P -2.319 -81.809 86.947 O3' -2.475 -84.220 92.101 17 u P -1.061 -84.968 92.013 O3' -1.483 -88.972 95.316 18 C P -0.062 -89.683 95.083 O3' -0.028 -94.858 95.663 19 U P 1.359 -95.477 95.142 O3' 1.666 -100.453 93.331 20 C P 3.099 -100.727 92.654 O3' 3.898 -103.951 88.862 21 C P 5.500 -103.877 88.721 O3' 7.574 -104.448 84.041 SG1 8 8 SG2 12 13 1 294.164 319.839 2 281.644 312.038 3 269.124 304.237 4 256.604 296.436 5 244.084 288.635 6 231.564 280.835 7 219.044 273.034 8 223.724 265.522 9 194.003 257.432 10 181.483 249.631 11 168.963 241.830 12 156.443 234.029 13 131.403 218.428 14 106.363 202.826 15 93.843 195.025 16 81.323 187.224 17 68.802 179.423 18 56.282 171.623 19 43.762 163.822 20 31.242 156.021 21 18.722 148.220 1 92 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 gGAGAGAGA AGUCAACCAG AGAAACACAC CAACCCAUUG CACUCCGGGU UGGUGGUAUA UUACCUGGUA CGGGGGAAAC UUCGUGGUGG CCG 1 GTP LOOP1 36 45 LOOP2 76 77 Crystallography ? ? Angstroms 1 g O3' 3.315 -90.868 77.425 2 G P 1.761 -91.193 77.696 O3' -0.637 -95.426 76.460 3 A P -2.119 -95.177 77.035 O3' -5.005 -98.740 78.907 4 G P -6.301 -97.838 79.205 O3' -8.954 -99.630 83.404 5 A P -10.156 -98.633 83.774 O3' -11.895 -98.572 88.575 6 G P -13.025 -97.471 88.882 O3' -13.867 -93.634 93.157 7 A P -15.171 -92.778 92.771 O3' -14.889 -87.756 94.232 8 G P -16.319 -87.310 93.656 O3' -16.186 -82.743 90.823 9 A P -17.456 -82.204 89.990 O3' -17.043 -77.600 87.793 10 A P -18.590 -77.523 87.391 O3' -19.675 -75.293 82.800 11 G P -21.273 -75.292 83.002 O3' -24.056 -76.808 78.702 12 U P -25.613 -76.800 79.109 O3' -28.671 -80.363 76.487 13 C P -30.144 -80.422 77.128 O3' -32.615 -85.175 77.590 14 A P -33.974 -85.195 78.449 O3' -36.127 -89.942 79.977 15 A P -35.869 -91.487 80.353 O3' -34.496 -94.722 83.520 16 C P -33.396 -95.639 82.775 O3' -32.002 -100.121 81.738 17 C P -30.710 -99.839 80.822 O3' -29.024 -102.813 77.110 18 A P -27.898 -101.834 76.515 O3' -27.070 -102.332 71.266 19 G P -26.183 -101.227 70.505 O3' -26.079 -100.206 65.555 20 A P -24.869 -99.149 65.486 O3' -25.362 -94.786 62.642 21 G P -24.086 -93.888 63.027 O3' -24.297 -88.904 63.881 22 A P -22.735 -88.564 64.004 O3' -22.520 -83.437 65.301 23 A P -21.302 -82.599 65.932 O3' -22.699 -78.229 68.486 24 A P -21.418 -77.487 69.107 O3' -21.445 -75.231 73.656 25 C P -19.944 -74.658 73.670 O3' -17.152 -75.006 77.785 26 A P -15.629 -74.522 77.687 O3' -10.669 -76.633 79.456 27 C P -9.707 -75.564 78.746 O3' -5.441 -77.990 77.145 28 A P -4.519 -76.909 76.404 O3' -1.188 -77.890 72.856 29 C P -0.600 -76.433 72.511 O3' 1.017 -75.416 67.599 30 C P 1.504 -73.888 67.531 O3' 0.990 -71.061 63.362 31 A P 1.362 -69.574 63.825 O3' -0.385 -65.539 61.237 32 A P 0.256 -64.266 61.976 O3' -2.236 -59.730 62.189 33 C P -1.243 -58.845 63.095 O3' -3.342 -55.594 66.509 34 C P -2.136 -55.082 67.449 O3' -3.227 -53.465 72.160 35 C P -1.823 -53.061 72.827 O3' -0.702 -53.485 77.735 36 A P 0.842 -53.115 77.921 O3' 3.919 -55.137 81.099 37 U P 5.352 -54.502 80.833 O3' 9.983 -56.971 80.860 38 U P 10.970 -55.718 80.640 O3' 16.153 -56.071 80.161 39 G P 16.430 -56.809 81.557 O3' 16.846 -62.148 81.786 40 C P 18.434 -62.330 81.979 O3' 19.434 -66.651 82.309 41 A P 20.111 -68.095 82.524 O3' 17.724 -71.698 79.452 42 C P 18.110 -73.118 80.096 O3' 19.123 -77.176 77.116 43 U P 19.089 -77.975 78.527 O3' 15.360 -77.700 76.066 44 C P 14.482 -76.361 76.277 O3' 10.405 -74.549 74.099 45 C P 10.276 -73.109 74.804 O3' 5.957 -70.728 76.366 46 G P 6.288 -69.490 77.343 O3' 0.799 -68.780 79.278 47 G P -0.595 -68.943 78.539 O3' -4.836 -66.424 80.008 48 G P -5.927 -66.918 78.941 O3' -9.720 -63.893 77.352 49 U P -10.624 -64.878 76.466 O3' -13.535 -63.019 72.636 50 U P -14.246 -64.333 72.046 O3' -15.283 -65.059 67.147 51 G P -15.673 -66.611 66.971 O3' -14.672 -69.149 62.563 52 G P -14.915 -70.730 62.747 O3' -12.307 -74.123 59.853 53 U P -12.945 -75.546 60.242 O3' -9.995 -79.852 60.557 54 G P -11.001 -81.019 61.004 O3' -8.671 -84.847 63.510 55 G P -9.749 -86.003 63.746 O3' -7.810 -89.682 67.014 56 U P -7.618 -90.512 68.365 O3' -12.425 -93.901 70.281 57 A P -13.299 -93.232 71.434 O3' -13.279 -89.220 68.799 58 U P -14.100 -89.137 67.421 O3' -17.575 -93.878 67.542 59 A P -19.000 -93.400 68.101 O3' -19.525 -94.506 73.238 60 U P -21.007 -94.841 73.753 O3' -24.692 -91.519 76.915 61 U P -25.929 -92.397 77.457 O3' -29.557 -89.925 75.621 62 A P -30.540 -89.212 74.552 O3' -34.106 -85.115 73.483 63 C P -35.346 -85.921 72.850 O3' -37.027 -86.280 68.279 64 C P -37.946 -87.579 68.471 O3' -38.651 -90.955 64.747 65 U P -39.607 -92.098 65.344 O3' -39.394 -96.770 63.671 66 G P -40.503 -97.616 64.463 O3' -40.340 -102.517 67.366 67 G P -41.813 -102.309 67.970 O3' -41.626 -105.535 72.733 68 U P -42.514 -105.436 74.065 O3' -41.658 -106.713 78.527 69 A P -43.155 -106.545 79.085 O3' -44.084 -105.177 83.683 70 C P -45.642 -104.952 83.987 O3' -48.085 -100.485 86.669 71 G P -49.550 -101.105 86.435 O3' -52.679 -96.390 85.624 72 G P -54.165 -96.982 85.483 O3' -57.331 -94.654 82.018 73 G P -58.690 -95.418 81.616 O3' -61.085 -94.258 77.191 74 G P -61.969 -95.554 76.850 O3' -62.940 -96.393 71.688 75 G P -64.124 -97.466 71.888 O3' -64.243 -101.595 69.621 76 A P -65.543 -102.294 70.258 O3' -63.700 -106.789 72.610 77 A P -62.165 -106.410 72.905 O3' -58.917 -110.514 71.240 78 A P -57.360 -110.143 71.408 O3' -53.500 -111.215 68.075 79 C P -52.196 -110.295 68.255 O3' -51.501 -104.871 67.680 80 U P -50.208 -103.945 67.923 O3' -50.692 -98.712 66.977 81 U P -49.420 -97.842 67.431 O3' -49.168 -92.913 69.023 82 C P -47.840 -92.622 69.879 O3' -47.484 -89.080 73.594 83 G P -45.970 -89.111 74.137 O3' -44.799 -87.966 79.109 84 U P -43.208 -87.807 78.923 O3' -40.793 -89.395 83.651 85 G P -39.568 -88.364 83.532 O3' -38.775 -89.836 88.419 86 G P -39.711 -88.852 89.276 O3' -39.774 -85.716 93.868 87 U P -41.006 -84.680 93.930 O3' -41.307 -79.702 96.952 88 G P -42.516 -78.681 96.697 O3' -42.304 -73.607 97.093 89 G P -43.606 -73.015 96.357 O3' -43.636 -68.510 94.020 90 C P -45.063 -68.226 93.342 O3' -46.473 -66.078 88.083 91 C P -48.072 -66.078 88.293 O3' -50.181 -68.541 84.717 92 G P -51.705 -68.934 84.391 O3' -54.089 -72.630 80.521 55 syn 57 syn 15 68 - - c 16 67 + + c 17 66 + + c 18 65 - - c 19 64 + + c 20 63 W W c 21 62 S H t 22 60 H W t 23 59 H W t 26 55 W W c 27 54 + + c 28 53 - - c 29 52 + + c 30 51 + + c 31 50 - - c 32 49 - - c 33 48 + + c 34 47 + + c 35 46 + + c 69 84 - - c 70 83 + + c 71 82 + + c 72 81 W W c 73 80 W W c 74 79 + + c 75 78 W H t 24 57 ! ! ! 27 55 ! ! ! 10 25 ! ! ! 11 24 ! ! ! 39 40 ! ! ! 43 44 ! ! ! 56 57 ! ! ! 26 55 10 22 60 2 15 68 7 69 84 7 SG1 9 10 SG2 14 14 SG3 24 25 SG4 36 45 SG5 56 58 SG6 61 61 SG7 76 77 SG8 92 92 1 0.000 178.268 2 12.520 186.069 3 25.040 193.870 4 37.560 201.671 5 50.080 209.472 6 62.600 217.273 7 75.121 225.073 8 87.641 232.874 9 100.161 240.675 10 125.201 256.277 11 150.241 271.878 12 162.761 279.679 13 175.281 287.480 14 170.601 294.992 15 181.070 411.644 16 181.070 396.893 17 181.070 382.141 18 181.070 367.390 19 181.070 352.638 20 181.070 337.887 21 181.070 323.135 22 181.070 293.633 23 181.070 278.881 24 181.070 249.378 25 181.070 219.875 26 181.070 190.372 27 181.070 175.621 28 181.070 160.869 29 181.070 146.118 30 181.070 131.366 31 181.070 116.615 32 181.070 101.863 33 181.070 87.112 34 181.070 72.360 35 181.070 57.609 36 191.086 46.871 37 195.234 32.786 38 192.635 18.334 39 183.840 6.575 40 170.710 0.000 41 156.026 0.000 42 142.897 6.575 43 134.102 18.334 44 131.503 32.786 45 135.651 46.871 46 145.667 57.609 47 145.667 72.360 48 145.667 87.112 49 145.667 101.863 50 145.667 116.615 51 145.667 131.366 52 145.667 146.118 53 145.667 160.869 54 145.667 175.621 55 145.667 190.372 56 145.667 205.124 57 145.667 234.627 58 145.667 264.130 59 145.667 278.881 60 145.667 293.633 61 145.667 308.384 62 145.667 323.135 63 145.667 337.887 64 145.667 352.638 65 145.667 367.390 66 145.667 382.141 67 145.667 396.893 68 145.667 411.644 69 145.667 441.147 70 145.667 455.899 71 145.667 470.650 72 145.667 485.402 73 145.667 500.153 74 145.667 514.905 75 145.667 529.656 76 152.619 550.000 77 174.118 550.000 78 181.070 529.656 79 181.070 514.905 80 181.070 500.153 81 181.070 485.402 82 181.070 470.650 83 181.070 455.899 84 181.070 441.147 85 200.321 303.082 86 212.842 310.883 87 225.362 318.684 88 237.882 326.484 89 250.402 334.285 90 262.922 342.086 91 275.442 349.887 92 282.954 354.568 1 91 + + c 2 90 + + c 3 89 + + c 4 88 + + c 5 87 - - c 6 86 + + c 7 85 + + c 9 13 + + c 10 12 - - c 11 11 + + c 12 9 S H t 13 25 + + c 15 7 W W c 16 6 + + c 17 5 - - c 18 4 + + c 19 3 - - c 20 2 + + c 21 1 + + c 14 8 ! ! ! 15 8 ! ! ! 1 91 7 9 13 3 14 8 8