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| Title: | | CRYSTAL STRUCTURE OF A HAIRPIN RIBOZYME IN THE CATALYTICALLY-ACTIVE CONFORMATION | |
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| Molecular Description: |
U1 SMALL NUCLEAR RIBONUCLEOPROTEIN/RNA INHIBITOR/RNA RIBOZYME COMPLEX |
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| Structural Features: | DOUBLE HELIX, LOOP, BULGES, FLIPPED-OUT BASES |
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| Nucleic Acid Sequence: |
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| Protein Sequence: |
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| Primary Citation: | Rupert, P.B., Massey, A.P., Sigurdsson, S.T., Ferre-D'Amare, A.R.
Transition state stabilization by a catalytic RNA
Science
, 298,
pp. 1421 - 1424, 2002.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 256.300 | b = 44.100 | c = 102.400 | (Ångstroms) |
 = 90.00 |  = 109.00 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, SITTING DROP |
| Drop: | | CaCl2, NH4Cl, MPD |
| Reservoir: | | CaCl2, NH4Cl, MPD |
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| Refinement: | The structure was refined using the CNS 1.0 program.
The R value is
22.9
for 41156 reflections
in the resolution range 61.07 to
2.40 Ångstroms
with I > 0.0 sigma(I).
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