1 21 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 GGCCACCUGA CaGUCCUCUC C 12 AVC Crystallography ? ? Angstroms 1 G O3' 110.171 53.102 3.953 2 G P 108.583 53.243 3.754 O3' 105.022 57.853 3.991 3 C P 103.546 57.492 3.475 O3' 100.083 60.369 1.138 4 C P 99.011 59.391 0.448 O3' 96.051 60.150 -3.458 5 A P 95.357 58.758 -3.870 O3' 93.815 58.134 -8.810 6 C P 93.131 56.684 -8.711 O3' 94.146 53.244 -12.534 7 C P 93.521 51.782 -12.298 O3' 95.229 47.429 -13.872 8 U P 94.162 46.383 -13.292 O3' 95.111 41.652 -11.955 9 G P 93.717 41.247 -11.270 O3' 94.013 38.005 -7.153 10 A P 92.674 37.733 -6.310 O3' 92.384 36.385 -1.533 11 C P 90.797 36.169 -1.476 O3' 88.183 37.739 2.686 12 a P 86.762 37.058 2.388 O3' 81.660 40.020 1.558 13 G O3' 75.436 42.133 3.748 14 U P 74.533 42.342 2.440 O3' 71.740 45.731 0.676 15 C P 70.956 44.731 -0.310 O3' 69.815 45.730 -5.210 16 C P 69.108 44.396 -5.757 O3' 69.552 42.778 -10.527 17 U P 68.798 41.370 -10.673 O3' 69.911 37.662 -13.689 18 C P 68.789 36.593 -13.280 O3' 69.869 31.618 -13.937 19 U P 68.598 30.776 -13.433 O3' 68.992 26.097 -11.582 20 C P 67.469 25.697 -11.268 O3' 66.254 22.671 -7.213 21 C P 64.666 22.621 -6.945 O3' 62.487 22.095 -2.422 13 syn SG1 1 1 SG2 8 8 SG3 12 13 1 21.715 326.423 2 29.893 323.038 3 43.524 317.398 4 57.155 311.758 5 70.785 306.118 6 84.416 300.478 7 98.046 294.837 8 101.431 303.016 9 125.308 283.557 10 138.938 277.917 11 152.569 272.277 12 166.200 266.636 13 193.461 255.356 14 220.722 244.075 15 234.353 238.435 16 247.983 232.795 17 261.614 227.155 18 275.245 221.515 19 288.875 215.874 20 302.506 210.234 21 316.136 204.594 1 92 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 GGAGAGAGA AGUCAACCAG AGAAACACAC CAACCCAUUG CACUCCGGGU UGGUGGUAUA UUACCUGGUA CGGGGGAAAC UUCGUGGUGG CCG LOOP1 36 45 LOOP2 76 77 Crystallography ? ? Angstroms 1 G O3' 65.322 35.904 4.775 2 G P 66.873 35.709 4.404 O3' 69.529 31.351 5.154 3 A P 71.057 31.626 4.730 O3' 74.033 28.104 2.873 4 G P 75.464 28.692 2.440 O3' 78.613 28.095 -2.356 5 A P 79.777 29.188 -2.482 O3' 81.423 30.643 -7.148 6 G P 82.382 31.936 -7.111 O3' 83.021 35.128 -10.858 7 A P 84.161 36.079 -10.246 O3' 83.711 41.032 -11.780 8 G P 85.028 41.683 -11.124 O3' 83.844 46.450 -9.096 9 A P 84.989 47.250 -8.306 O3' 83.748 51.703 -6.065 10 A P 85.212 52.159 -5.583 O3' 85.808 54.342 -0.912 11 G P 87.399 54.574 -0.940 O3' 90.063 53.229 3.422 12 U P 91.639 53.365 3.141 O3' 95.034 50.187 5.664 13 C P 96.476 50.450 5.000 O3' 99.681 46.187 4.398 14 A P 101.089 46.530 3.688 O3' 103.482 41.694 1.599 15 A P 105.080 41.659 1.462 O3' 102.971 38.137 -1.500 16 C P 101.756 37.637 -0.575 O3' 100.151 32.741 -0.530 17 C P 98.914 32.669 0.490 O3' 97.687 28.934 3.624 18 A P 96.538 29.472 4.611 O3' 95.731 27.995 9.387 19 G P 94.615 28.919 10.083 O3' 94.270 29.391 15.154 20 A P 93.089 30.427 15.487 O3' 93.236 35.122 18.839 21 G P 91.772 35.761 18.692 O3' 91.436 40.719 17.686 22 A P 89.835 40.893 17.746 O3' 88.839 46.081 16.625 23 A P 87.564 46.795 15.964 O3' 88.419 51.423 13.511 24 A P 87.080 51.923 12.768 O3' 87.164 54.214 8.181 25 C P 85.633 54.680 8.223 O3' 82.773 53.913 3.856 26 A P 81.220 54.281 4.061 O3' 77.412 51.715 1.928 27 C P 76.127 52.461 2.536 O3' 71.946 49.863 4.117 28 A P 70.888 50.854 4.808 O3' 67.580 49.547 8.599 29 C P 66.830 50.916 8.974 O3' 65.314 51.550 13.916 30 C P 64.743 53.041 14.098 O3' 64.822 55.604 18.432 31 A P 64.236 57.060 18.108 O3' 65.339 61.106 20.666 32 A P 64.502 62.293 19.973 O3' 66.102 66.908 20.215 33 C P 65.185 67.900 19.356 O3' 66.787 71.908 15.674 34 C P 65.508 72.368 14.823 O3' 66.563 74.230 10.140 35 C P 65.107 74.564 9.552 O3' 64.140 73.985 4.528 36 A P 62.583 74.348 4.424 O3' 59.602 71.757 1.104 37 U P 58.168 72.385 1.425 O3' 53.852 69.467 1.382 38 U P 52.782 70.643 1.617 O3' 47.709 69.822 2.094 39 G P 47.482 69.045 0.703 O3' 47.632 63.630 0.470 40 C P 46.072 63.225 0.365 O3' 44.490 58.388 0.868 41 A P 45.206 57.450 -0.218 O3' 47.998 54.028 2.591 42 C P 47.815 52.608 1.862 O3' 47.513 48.411 4.891 43 U P 47.623 47.410 3.639 O3' 52.200 48.035 5.493 44 C P 52.142 49.637 5.628 O3' 55.863 52.048 8.189 45 C P 55.594 53.496 7.556 O3' 59.547 56.271 5.761 46 G P 59.132 57.492 4.792 O3' 64.341 58.765 2.566 47 G P 65.767 58.620 3.298 O3' 69.586 61.734 1.985 48 G P 70.798 61.327 2.954 O3' 74.165 64.695 4.604 49 U P 75.210 63.825 5.457 O3' 77.794 65.921 9.348 50 U P 78.689 64.727 9.944 O3' 79.812 64.136 14.898 51 G P 80.440 62.656 14.970 O3' 79.838 59.844 19.289 52 G P 80.425 58.373 19.022 O3' 78.159 54.364 21.487 53 U P 78.984 53.042 21.098 O3' 76.575 48.419 21.081 54 G P 77.613 47.325 20.511 O3' 75.778 43.199 18.010 55 G P 76.945 42.151 17.646 O3' 75.762 38.149 14.412 56 U P 75.013 37.390 13.216 O3' 80.260 35.119 11.056 57 A P 80.977 35.641 9.729 O3' 80.669 39.311 12.512 58 U P 81.535 39.408 13.863 O3' 85.227 34.940 13.128 59 A P 86.594 35.572 12.566 O3' 87.372 35.266 7.454 60 U P 88.932 34.892 7.454 O3' 92.040 38.342 4.128 61 U P 93.638 38.258 3.992 O3' 97.477 41.542 5.605 62 A P 98.008 41.184 7.080 O3' 100.977 45.521 8.452 63 C P 102.252 44.781 9.098 O3' 103.625 44.411 14.026 64 C P 104.685 43.205 14.139 O3' 105.210 39.747 17.708 65 U P 106.524 39.043 17.106 O3' 107.164 34.000 18.255 66 G P 108.399 33.281 17.528 O3' 108.878 28.634 14.211 67 G P 110.293 28.713 13.460 O3' 110.229 25.965 8.368 68 U P 111.337 26.238 7.241 O3' 110.557 25.701 2.326 69 A P 112.005 26.082 1.745 O3' 112.811 28.389 -2.646 70 C P 114.412 28.323 -2.537 O3' 118.044 32.665 -4.023 71 G P 119.469 32.922 -3.329 O3' 121.857 37.072 -2.291 72 G P 123.193 36.670 -1.492 O3' 125.671 39.802 1.554 73 G P 126.860 38.717 1.605 O3' 128.707 38.498 6.026 74 G P 129.884 37.417 6.194 O3' 131.666 35.134 10.338 75 G P 132.554 33.879 9.865 O3' 133.147 29.490 12.386 76 A P 134.424 29.322 11.422 O3' 131.637 25.412 8.140 77 A P 130.147 25.926 7.811 O3' 126.418 21.988 8.493 78 A P 124.891 22.490 8.524 O3' 121.399 21.624 12.218 79 C P 120.287 22.485 11.431 O3' 118.967 26.498 14.900 80 U P 117.803 27.533 14.511 O3' 118.472 32.372 16.408 81 U P 117.426 33.526 16.021 O3' 118.172 38.797 13.740 82 C P 116.621 39.197 13.823 O3' 115.242 43.195 9.860 83 G P 113.737 43.191 9.301 O3' 112.899 44.649 4.411 84 U P 111.371 44.584 3.917 O3' 109.289 42.694 -1.225 85 G P 108.518 44.104 -1.213 O3' 107.436 44.604 -6.602 86 G P 108.128 45.941 -7.155 O3' 107.448 47.162 -12.182 87 U P 108.388 48.463 -12.254 O3' 107.375 52.736 -14.876 88 G P 108.565 53.767 -14.553 O3' 107.673 59.010 -14.912 89 G P 108.806 59.912 -14.214 O3' 107.405 64.465 -11.857 90 C P 108.685 65.112 -11.128 O3' 107.494 68.075 -6.757 91 C P 108.664 68.729 -5.865 O3' 109.626 68.838 -0.054 92 G P 110.913 69.752 0.267 O3' 113.244 65.204 -0.523 57 syn 73 syn 76 syn 15 68 - - c 16 67 + + c 17 66 + + c 18 65 - - c 19 64 + + c 20 63 W W c 21 62 S H t 22 60 H W t 23 59 H W t 26 55 W W c 27 54 + + c 28 53 - - c 29 52 + + c 30 51 + + c 31 50 - - c 32 49 - - c 33 48 + + c 34 47 + + c 35 46 + + c 69 84 - - c 70 83 + + c 71 82 + + c 72 81 W W c 74 79 + + c 75 78 S H t 24 57 ! ! ! 27 55 ! ! ! 69 83 ! ! ! 73 80 ! ! ! 10 25 ! ! ! 11 24 ! ! ! 15 85 ! ! ! 39 40 ! ! ! 43 44 ! ! ! 56 57 ! ! ! 75 77 ! ! ! 26 55 10 22 60 2 15 68 7 69 84 7 SG1 9 10 SG2 14 14 SG3 24 25 SG4 36 45 SG5 56 58 SG6 61 61 SG7 76 77 SG8 91 92 1 302.600 171.880 2 288.969 177.521 3 275.339 183.161 4 261.708 188.801 5 248.077 194.441 6 234.447 200.082 7 220.816 205.722 8 207.186 211.362 9 193.555 217.002 10 166.294 228.283 11 139.032 239.563 12 125.402 245.203 13 111.771 250.843 14 108.387 242.665 15 166.927 411.644 16 166.927 396.893 17 166.927 382.141 18 166.927 367.390 19 166.927 352.638 20 166.927 337.887 21 166.927 323.135 22 166.927 293.633 23 166.927 278.881 24 166.927 249.378 25 166.927 219.875 26 166.927 190.372 27 166.927 175.621 28 166.927 160.869 29 166.927 146.118 30 166.927 131.366 31 166.927 116.615 32 166.927 101.863 33 166.927 87.112 34 166.927 72.360 35 166.927 57.609 36 176.943 46.871 37 181.091 32.786 38 178.492 18.334 39 169.697 6.575 40 156.567 0.000 41 141.884 0.000 42 128.754 6.575 43 119.959 18.334 44 117.360 32.786 45 121.508 46.871 46 131.524 57.609 47 131.524 72.360 48 131.524 87.112 49 131.524 101.863 50 131.524 116.615 51 131.524 131.366 52 131.524 146.118 53 131.524 160.869 54 131.524 175.621 55 131.524 190.372 56 131.524 205.124 57 131.524 234.627 58 131.524 264.130 59 131.524 278.881 60 131.524 293.633 61 131.524 308.384 62 131.524 323.135 63 131.524 337.887 64 131.524 352.638 65 131.524 367.390 66 131.524 382.141 67 131.524 396.893 68 131.524 411.644 69 131.524 441.147 70 131.524 455.899 71 131.524 470.650 72 131.524 485.402 73 131.524 500.153 74 131.524 514.905 75 131.524 529.656 76 138.476 550.000 77 159.975 550.000 78 166.927 529.656 79 166.927 514.905 80 166.927 500.153 81 166.927 485.402 82 166.927 470.650 83 166.927 455.899 84 166.927 441.147 85 84.510 262.124 86 70.879 267.764 87 57.249 273.404 88 43.618 279.045 89 29.987 284.685 90 16.357 290.325 91 8.178 293.709 92 0.000 297.093 2 90 + + c 3 89 + + c 4 88 + + c 5 87 - - c 6 86 + + c 7 85 + + c 9 13 + + c 10 12 - - c 11 11 + + c 12 9 S H t 13 25 + + c 15 7 W W c 16 6 + + c 17 5 - - c 18 4 + + c 19 3 - - c 20 2 + + c 21 1 + + c 14 8 ! ! ! 2 90 6 9 13 3 14 8 8