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| Title: | | CRYSTAL STRUCTURE OF TRNA PSEUDOURIDINE SYNTHASE TRUB AND ITS RNA COMPLEX: RNA-PROTEIN RECOGNITION THROUGH A COMBINATION OF RIGID DOCKING AND INDUCED FIT | |
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| Molecular Description: |
tRNA pseudouridine synthase B (E.C. 4.2.1.70)/RNA Complex |
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| Structural Features: | RH DOUBLE HELIX, LOOPS, HAIRPIN LOOP, BULGES, FLIPPED-OUT BASES, |
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| Nucleic Acid Sequence: |
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| Chain | C: | |
C U G U G U (FHU) C G A U C C A C A G
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| Chains | D, E: | |
C U G U G U U C G A U C C A C A G
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| Protein Sequence: |
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| Primary Citation: | Pan, H., Agarwalla, S., Moustakas, D.T., Finer-Moore, J., Stroud, R.M.
Crystal Structure of tRNA Pseudouridine Synthase TruB and Its RNA Complex: RNA Recognition Through a Combination of Rigid Docking and Induced Fit
Proc.Natl.Acad.Sci.USA
, 100,
pp. 12648 - 12653, 2003.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
C
1
2
1
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| Cell Constants: |
| a = 98.862 | b = 159.337 | c = 44.545 | (Ångstroms) |
 = 90.00 |  = 97.77 |  = 90.00 | (degrees) |
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| Crystallization Conditions: |
| Method: | | VAPOR DIFFUSION, HANGING DROP |
| Drop: | | ammonium sulfate, magnesium sulfate, sodium cacodylate, H2O |
| Reservoir: | | ammonium sulfate, magnesium sulfate, sodium cacodylate, H2O |
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| Refinement: | The structure was refined using the CNS program.
The R value is
22.3
for 38642 reflections
in the resolution range 15 to
2.1 Ångstroms
with Fobs > 2.1 sigma(Fobs).
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