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| Title: | | MECHANISM OF CCA-ADDING ENZYMES SPECIFICITY REVEALED BY CRYSTAL STRUCTURES OF TERNARY COMPLEXES | |
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| Molecular Description: |
tRNA nucleotidyltransferase (E.C.2.7.7.25)/RNA Complex |
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| Structural Features: | T SINGLE STRAND, INTERNAL LOOP, HAIRPIN LOOP |
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| Nucleic Acid Sequence: |
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| Chains | E,F: | | G C G G A U U U A (2MG) C U C A G (H2U) (H2U) G G G A G A G C (M2G) C C A G A (OMC) U (OMG) A A (YG) A (PSU) (5MC) U G G A G (7MG) U C (5MC) U G U G (5MU) (PSU) C G (1MA) U C C A C A G A A U U C G C A C C A |
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| Protein Sequence: |
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| Primary Citation: | Xiong, Y., Steitz, T.A.
Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template.
Nature
, 430,
pp. 640 - 645, 2004.
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| Experimental Information: | X-RAY DIFFRACTION |
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| Space Group: |
P
3
2
1
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| Cell Constants: |
| a = 218.403 | b = 218.403 | c = 170.263 | (Ångstroms) |
 = 90.00 |  = 90.00 |  = 120.00 | (degrees) |
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| Refinement: | The structure was refined using the REFMAC 5.2.0005 program.
The R value is
36.0
for 10111 reflections
in the resolution range 44.72 to
6.21 Ångstroms
. |
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